7EVT
Crystal structure of the N-terminal degron-truncated human glutamine synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-11 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.917, 158.866, 118.908 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 44.430 - 2.950 |
| R-factor | 0.2206 |
| Rwork | 0.219 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qc8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.566 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19.4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.130 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.078 | 0.772 |
| Number of reflections | 91909 | 14650 |
| <I/σ(I)> | 20.24 | 3.09 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 7.6 | 7.6 |
| CC(1/2) | 0.990 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293.15 | 0.07 M sodium citrate (pH 5.1), 10% glycerol and 5.6% PEG4000 |
