7EVL

Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 64% GTP/36% GDP and 2 Na+ in a small unit cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-11
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.166, 79.515, 90.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.150
Rwork	0.203
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7evc
RMSD bond length	0.009
RMSD bond angle	0.914
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.077	0.555
Rmeas	0.083	0.622
Rpim	0.031	0.273
Number of reflections	16163	669
<I/σ(I)>	22.3	1.3
Completeness [%]	98.1
Redundancy	6.7
CC(1/2)		0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	25% PEG 1500, 0.1 M MMT, pH 9.0, soaked with 10 mM GTP 1 hr, back soaked 22h with no GTP