7EVH
Odinarchaeota tubulin H393D mutant, in a psuedo protofilament arrangement, bound to 59% GDP, 41% phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-08-16 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.300, 93.342, 100.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.800 - 2.500 |
Rwork | 0.199 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o2r |
RMSD bond length | 0.005 |
RMSD bond angle | 0.564 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (v1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.114 | 0.544 |
Rmeas | 0.124 | 0.660 |
Rpim | 0.047 | 0.364 |
Number of reflections | 13455 | 526 |
<I/σ(I)> | 14.7 | 1.5 |
Completeness [%] | 98.0 | |
Redundancy | 6.4 | |
CC(1/2) | 0.699 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 1500, 0.1 M SPG (2 succinic acid: 7 sodium dihydrogen phosphate), pH 9.0 |