7EVG

Apo Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a psuedo-protofilament arrangement

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.102, 94.184, 100.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.400 - 2.480
Rwork	0.211
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o2r
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.100	2.570
High resolution limit [Å]	2.480	2.480
Rmerge	0.185	1.230
Rmeas	0.201	1.340
Rpim	0.078	0.516
Number of reflections	14154	1525
<I/σ(I)>	8.6	1.7
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)		0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 1500, 0.1 M SPG, pH 7.0, soaked with 100 mM phosphate for 2 h