7EVF

Plant growth-promoting factor YxaL mutant from Bacillus velezensis - T175W/S213G/W215A

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100.15
Detector technology	CCD
Collection date	2020-03-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00003
Spacegroup name	H 3
Unit cell lengths	152.987, 152.987, 61.694
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.880 - 1.500
R-factor	0.1505
Rwork	0.150
R-free	0.16480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dxn
RMSD bond length	0.010
RMSD bond angle	1.083
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rmeas		0.320
Rpim	0.020	0.116
Number of reflections	84369	4087
<I/σ(I)>	33	4.9
Completeness [%]	98.1
Redundancy	8.3
CC(1/2)	0.998	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	0.2 M sodium citrate pH 5.8, 16% (w/v) PEG 4000, 20% (v/v) Isopropanol