7EVD

Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 53% GTP/47% and 2 Na+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-11
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.361, 92.104, 102.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 1.450
Rwork	0.152
R-free	0.17620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7evc
RMSD bond length	0.015
RMSD bond angle	1.535
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.114	0.593
Rmeas	0.119	0.723
Rpim	0.035	0.387
Number of reflections	67867	2897
<I/σ(I)>	34.2	1
Completeness [%]	98.8
Redundancy	10.9
CC(1/2)		0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 1500, 0.1 M MMT (1 DL-malic acid: 2 MES: 2 Tris base), pH 9.0, soaked with 10 mM GTP for 2 h, before back soaking without GTP (70 h), before freezing