7EVB

Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 77% GTP/23% and 2 Na+

Replaces:  7DON

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-23
Detector	RAYONIX MX-300
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	40.327, 92.256, 103.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.410 - 1.620
Rwork	0.153
R-free	0.17700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o2r
RMSD bond length	0.006
RMSD bond angle	0.912
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.050	0.479
Rmeas	0.054	0.544
Rpim	0.020	0.251
Number of reflections	48739	2085
<I/σ(I)>	27.6	1.6
Completeness [%]	97.9	85.6
Redundancy	6.5	3.7
CC(1/2)		0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	20% PEG 3350, 0.2 M sodium acetate, 0.1 M Bis-Tris propane, pH 8.5, soaked with 10 mM GTP for 1 h before freezing