7EVA
Structure of molecular chaperone SycE of Yersinia enterocolitica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-25 |
Detector | APEX II CCD |
Wavelength(s) | 1.5 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 58.000, 58.000, 77.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.938 - 2.083 |
R-factor | 0.201 |
Rwork | 0.200 |
R-free | 0.23450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n5b |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS (1.15.2_3472) |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2-3472-000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.940 | 2.140 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.200 | 0.278 |
Number of reflections | 8342 | 8342 |
<I/σ(I)> | 1.51 | |
Completeness [%] | 99.7 | |
Redundancy | 15.11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 100mM MES pH 6.0, 50-80mM Na-Citrate, 5-10% PEG 3350, 100mM NaCl |