7EUK
Crystal structure of N(omega)-hydroxy-L-arginine hydrolase in complex with L-Orn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-24 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.392, 46.963, 59.317 |
| Unit cell angles | 83.53, 84.71, 70.14 |
Refinement procedure
| Resolution | 44.000 - 1.400 |
| R-factor | 0.1591 |
| Rwork | 0.158 |
| R-free | 0.17910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6luh |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.000 | 44.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.540 | 1.400 |
| Rmerge | 0.053 | 0.040 | 0.403 |
| Total number of observations | 652069 | 3340 | 27040 |
| Number of reflections | 88405 | 591 | 4428 |
| <I/σ(I)> | 22.6 | 55.7 | 3.8 |
| Completeness [%] | 96.0 | 99.5 | 93.5 |
| Redundancy | 7.4 | 5.7 | 6.1 |
| CC(1/2) | 0.999 | 0.998 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 298 | PEG 4000, MgCl2 |
