7ETX

Crystal structure of bifunctional indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase (TrpC) from corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	90.681, 90.681, 141.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.740 - 2.100
R-factor	0.1954
Rwork	0.193
R-free	0.25200
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.522
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.083	0.061	0.345
Rmeas	0.087	0.063	0.376
Rpim	0.024	0.018	0.139
Total number of observations	352134
Number of reflections	34548	1888	1664
<I/σ(I)>	13.9
Completeness [%]	98.0	95.8	96.6
Redundancy	10.2	12.8	5.5
CC(1/2)		0.998	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	0.8M Pottasium sodium tartrate tetrahydrate, 0.1M Sodium HEPES pH 7.5