7ET5
Crystal structure of Arabidopsis TEM1 AP2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.650, 41.200, 59.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.958 - 1.052 |
| R-factor | 0.1641 |
| Rwork | 0.164 |
| R-free | 0.17390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcc |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.050 | 0.791 |
| Rmeas | 0.055 | 0.881 |
| Rpim | 0.022 | 0.378 |
| Number of reflections | 42719 | 3611 |
| <I/σ(I)> | 24.2 | 1.2 |
| Completeness [%] | 96.2 | 83 |
| Redundancy | 6.2 | 4.9 |
| CC(1/2) | 1.000 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 0.2 M Li2SO4, 0.1 M Tris pH 8.5, 18% PEG 8000 |
