7ET4

Crystal structure of Arabidopsis TEM1 AP2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	107.316, 135.155, 66.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.524 - 2.700
R-factor	0.2043
Rwork	0.203
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gcc
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.140	0.819
Rmeas	0.160	0.947
Rpim	0.076	0.464
Number of reflections	27364	2704
<I/σ(I)>	7.4	1.25
Completeness [%]	99.9	100
Redundancy	4.3	4.1
CC(1/2)	0.984	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.2 M NH4Cl, 0.1 M NaAc pH 5.0, 20% PEG 6000