7ESW

Root induced Secreted protein Tsp1 from Biocontrol fungi Trichoderma virens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-02-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	47.290, 48.720, 56.200
Unit cell angles	90.00, 112.35, 90.00

Refinement procedure

Resolution	31.950 - 1.250
R-factor	0.152
Rwork	0.151
R-free	0.17450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cwj
RMSD bond length	0.011
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.054	0.497
Rmeas	0.060	0.554
Rpim	0.025	0.240
Number of reflections	64807	3121
<I/σ(I)>	17
Completeness [%]	99.0
Redundancy	5.5
CC(1/2)	0.997	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	1.9M Sodium malonate pH 6.5, 5% Glycerol