7ESS

Structure-guided studies of the Holliday junction resolvase RuvX provide novel insights into ATP-stimulated cleavage of branched DNA and RNA substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-03-28
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	117.490, 42.580, 58.110
Unit cell angles	90.00, 114.45, 90.00

Refinement procedure

Resolution	52.950 - 1.930
R-factor	0.183
Rwork	0.181
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vhx
RMSD bond length	0.012
RMSD bond angle	1.484
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.477	53.477	2.040
High resolution limit [Å]	1.930	6.110	1.930
Rmerge		0.036	0.325
Rmeas	0.075	0.042	0.368
Rpim	0.034	0.021	0.171
Number of reflections	19756	675	2772
<I/σ(I)>	13.2	9.5	2.2
Completeness [%]	99.3	99.9	96.9
Redundancy	4.7	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	300	0.1 M Bis-Tris propane, pH 7.0 and 2.4 M sodium malonate, pH 7.0