7ESF
The Crystal Structure of human MTH1 from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.521200 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 36.276, 60.533, 66.135 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.653 - 1.550 |
| Rwork | 0.189 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q93 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.867 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.653 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.076 | 0.427 |
| Number of reflections | 21691 | 1011 |
| <I/σ(I)> | 28.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 0.1 M Citric Acid pH 4.0, 30% PEG 6000 |
