7ESA
the complex structure of flavin transferase FmnB complexed with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.089, 67.294, 86.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.280 - 1.800 |
| Rwork | 0.194 |
| R-free | 0.22270 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.449 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (v1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.280 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.119 | |
| Number of reflections | 36579 | 3603 |
| <I/σ(I)> | 48.17 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.9 | |
| CC(1/2) | 0.971 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 286 | 0.2 M Potassium sodium tartrate tetrahydrate, 20% w/v Polyethylene glycol 3350 |
