7EQZ
Crystal structure of Carboxypeptidase B complexed with Potato Carboxypeptidase Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-09-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.451, 87.208, 49.069 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 31.890 - 2.200 |
| R-factor | 0.1586 |
| Rwork | 0.154 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cpa |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.502 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.890 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 15975 | 1009 |
| <I/σ(I)> | 48.29 | |
| Completeness [%] | 96.6 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.6M Ammonium sulfate, 0.1M Sodium HEPES pH 7.5, 0.1M Sodium chloride |
