7EQM

Apo Truncated VhChiP (Delta 1-19)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2020-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	105.244, 151.388, 95.827
Unit cell angles	90.00, 111.12, 90.00

Refinement procedure

Resolution	20.003 - 2.500
R-factor	0.180844482218
Rwork	0.178
R-free	0.22517
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mdo
RMSD bond length	0.007
RMSD bond angle	0.857
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.003	7.980
High resolution limit [Å]	2.500	2.500
Rmeas	0.119
Rpim	0.061
Number of reflections	43095	43095
<I/σ(I)>	7.3
Completeness [%]	89.4
Redundancy	3.1
CC(1/2)	0.868	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	292	0.1 M Lithium sulfate, 0.1 M ADA pH 5.2, and 26% v/v PEG 400