7EQE

Crystal Structure of a transcription factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97899
Spacegroup name	C 1 2 1
Unit cell lengths	163.790, 53.130, 102.940
Unit cell angles	90.00, 104.50, 90.00

Refinement procedure

Resolution	47.943 - 2.399
R-factor	0.2418
Rwork	0.240
R-free	0.26740
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.943	2.540
High resolution limit [Å]	2.399	2.399
Number of reflections	65417	10421
<I/σ(I)>	7.39
Completeness [%]	99.4
Redundancy	3.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	HEPES-NaOH, polyethylene glycol 4000, isopropanol