7EP4

Crystal structure of ZER1 bound to GFLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-12-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.979191
Spacegroup name	P 21 21 21
Unit cell lengths	59.940, 70.150, 148.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.430 - 2.070
Rwork	0.179
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep0
RMSD bond length	0.007
RMSD bond angle	0.842
Data reduction software	XDS (0.7.4)
Data scaling software	XDS (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.430	2.120
High resolution limit [Å]	2.066	2.070
Rmerge	0.258	0.627
Number of reflections	38879	2661
<I/σ(I)>	19.5	8.5
Completeness [%]	99.3
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Ammonium formate, 20% (wt/vol) Polyethylene glycol 3,350