7EOZ
The structure of rice Defective Pollen Wall (DPW) in the complex with its cofactor NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-03-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 61 |
| Unit cell lengths | 222.814, 222.814, 114.024 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.450 - 3.400 |
| R-factor | 0.1938 |
| Rwork | 0.191 |
| R-free | 0.23960 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
| Rmerge | 0.136 | 0.057 | 0.788 |
| Total number of observations | 540754 | ||
| Number of reflections | 48784 | 2422 | 2411 |
| <I/σ(I)> | 16 | ||
| Completeness [%] | 99.8 | 95.7 | 100 |
| Redundancy | 11.1 | 9.4 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 2.8 M sodium formate, pH 7.0, 0.1 M magnesium chloride hexahydrate, 0.1 M HEPES, pH 7.5, 5% (w/v) PEG 3350 |
