7EOX

Protease structure from Euphorbia resinifera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.90000
Spacegroup name	P 21 21 21
Unit cell lengths	67.673, 112.880, 201.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.010 - 1.700
R-factor	0.20434
Rwork	0.203
R-free	0.23667
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.621
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.160	64.160	1.730
High resolution limit [Å]	1.700	9.310	1.700
Rmerge	0.116	0.031	2.683
Rmeas	0.141	0.038	3.278
Rpim	0.080	0.021	1.853
Number of reflections	168106	1029	8027
<I/σ(I)>	9.7
Completeness [%]	99.0
Redundancy	5.8	5.8	6
CC(1/2)	0.995	0.972	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.2M sodium idodide (0.2 M), 0.1M Bis-Tris propane pH 8.5, 20% W/V PEG 3350