7EMD
Mooring Stone-Like Arg114 Pulls Diverse Bulged Peptides: First Insight into African Swine Fever Virus-Derived T Cell Epitopes Presented by Swine Major Histocompatibility Complex Class I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03836 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.320, 110.810, 45.989 |
| Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
| Resolution | 44.950 - 1.700 |
| R-factor | 0.2106 |
| Rwork | 0.208 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.870 |
| Data scaling software | HKL-2000 (1.13_2998) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.098 | 0.319 |
| Number of reflections | 43514 | 43514 |
| <I/σ(I)> | 5.71 | |
| Completeness [%] | 98.0 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | no |
