7EMD

Mooring Stone-Like Arg114 Pulls Diverse Bulged Peptides: First Insight into African Swine Fever Virus-Derived T Cell Epitopes Presented by Swine Major Histocompatibility Complex Class I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03836
Spacegroup name	P 1 21 1
Unit cell lengths	41.320, 110.810, 45.989
Unit cell angles	90.00, 102.22, 90.00

Refinement procedure

Resolution	44.950 - 1.700
R-factor	0.2106
Rwork	0.208
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qq3
RMSD bond length	0.007
RMSD bond angle	0.870
Data scaling software	HKL-2000 (1.13_2998)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	1.700	1.700
Rmerge	0.098	0.319
Number of reflections	43514	43514
<I/σ(I)>	5.71
Completeness [%]	98.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	no