7EMB
Mooring Stone-Like Arg114 Pulls Diverse Bulged Peptides: First Insight into African Swine Fever Virus-Derived T Cell Epitopes Presented by Swine Major Histocompatibility Complex Class I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.745, 111.407, 46.042 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 25.090 - 1.970 |
| R-factor | 0.2208 |
| Rwork | 0.218 |
| R-free | 0.28320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.850 |
| Data scaling software | HKL-2000 (1.13_2998) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.700 | 2.070 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.117 | 0.414 |
| Number of reflections | 29130 | 29130 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M Ammonium acetate,0.1 M BIS-TRIS pH 5.5,17% w/v Polyethylene glycol 10,000 |
