7EMA
Mooring Stone-Like Arg114 Pulls Diverse Bulged Peptides: First Insight into African Swine Fever Virus-Derived T Cell Epitopes Presented by Swine Major Histocompatibility Complex Class I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2019-12-24 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.03997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.391, 107.350, 46.105 |
| Unit cell angles | 90.00, 101.72, 90.00 |
Refinement procedure
| Resolution | 26.820 - 1.800 |
| R-factor | 0.1855 |
| Rwork | 0.183 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.836 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.529 |
| Number of reflections | 36183 | 36183 |
| <I/σ(I)> | 5.35 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M HEPES pH 7.5, 30% w/v Polyethylene glycol 1,000. |
