7EM9
Mooring Stone-Like Arg114 Pulls Diverse Bulged Peptides: First Insight into African Swine Fever Virus-Derived T Cell Epitopes Presented by Swine Major Histocompatibility Complex Class I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.558, 114.756, 46.025 |
| Unit cell angles | 90.00, 102.63, 90.00 |
Refinement procedure
| Resolution | 27.280 - 1.900 |
| R-factor | 0.1988 |
| Rwork | 0.196 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.914 |
| Data scaling software | HKL-2000 (1.13_2998) |
| Phasing software | PHASER (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.664 |
| Number of reflections | 29020 | 29020 |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 92.5 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M BIS-TRIS propane pH 9.0, 30% w/v Polyethylene glycol 6,000 |
