7ELX

The crystal structure of CTLA-4 and Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	95.923, 196.921, 148.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.200 - 2.140
R-factor	0.1778
Rwork	0.177
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rp8
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.640	2.180
High resolution limit [Å]	2.140	2.140
Rmerge	0.101	0.101
Number of reflections	76857
<I/σ(I)>	27.12
Completeness [%]	100.0
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	PEG8000 10%, 0.1M Na Citrate 0.1M (NH4)2SO4