7EL6

Structure of SMCR8 bound FEM1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97853
Spacegroup name	P 21 21 2
Unit cell lengths	83.306, 91.497, 112.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.383 - 2.802
R-factor	0.2091
Rwork	0.203
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.383	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	20988	1998
<I/σ(I)>	49.5
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Tris pH7.3 4% PEG8000