7EKX

Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A E564Q) in complex with maltononaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	160.654, 206.280, 258.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.150 - 3.400
R-factor	0.2739
Rwork	0.272
R-free	0.30340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d0f
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.160	3.490
High resolution limit [Å]	3.400	3.400
Number of reflections	58913	4334
<I/σ(I)>	8.31
Completeness [%]	99.6
Redundancy	7.3
CC(1/2)	0.998	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride