7EKW

Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (D535N) in complex with maltotetrose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97939
Spacegroup name	C 1 2 1
Unit cell lengths	160.250, 198.940, 134.590
Unit cell angles	90.00, 104.87, 90.00

Refinement procedure

Resolution	25.260 - 3.100
R-factor	0.2493
Rwork	0.248
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d0f
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.750	3.270
High resolution limit [Å]	3.100	3.100
Number of reflections	73585	10713
<I/σ(I)>	6.7
Completeness [%]	99.8	99.9
Redundancy	3.7
CC(1/2)	0.991	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride