7EKU

Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W958A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	160.221, 200.651, 259.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 3.100
R-factor	0.2326
Rwork	0.231
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d06
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.800	46.800	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.100	0.036	0.950
Rmeas	0.107	0.039	1.020
Total number of observations	562556
Number of reflections	75998	912	5569
<I/σ(I)>	15.39	36.57	1.95
Completeness [%]	99.9	94.8	100
Redundancy	7.402	6.127	7.555
CC(1/2)	0.998	0.997	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride