7EK8

Crystal structure of apo streptavidin at ambient temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE MFX
Synchrotron site	SLAC LCLS
Beamline	MFX
Temperature [K]	298
Detector technology	PIXEL
Collection date	2020-08-16
Detector	SLAC ePix10k 2M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	47.400, 87.700, 58.900
Unit cell angles	90.00, 98.90, 90.00

Refinement procedure

Resolution	48.488 - 1.700
R-factor	0.1917
Rwork	0.190
R-free	0.22420
Structure solution method	FOURIER SYNTHESIS
Data reduction software	CrystFEL
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_3318)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.490	48.490
High resolution limit [Å]	1.700	1.700
Number of reflections	52270
<I/σ(I)>	7.66
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.960	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Pact PremierTM 100 mM MMT buffer pH 6.0 and 25 % w/v PEG 1500