7EJV
The co-crystal structure of DYRK2 with YK-2-69
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 277 |
Detector technology | PIXEL |
Collection date | 2020-07-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.860, 129.387, 291.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 2.500 |
R-factor | 0.2021 |
Rwork | 0.201 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lxd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.302 |
Data reduction software | HKL-2000 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.860 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 40357 | 3967 |
<I/σ(I)> | 14.02 | |
Completeness [%] | 99.7 | 99.97 |
Redundancy | 13.3 | |
CC(1/2) | 0.998 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.1 M sodium citrate pH 5.5, 8% PEG3350 |