7EIJ

Ancestral L-Lys oxidase K387A variant (L-Arg binding form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-18
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	85.932, 80.093, 88.036
Unit cell angles	90.00, 90.47, 90.00

Refinement procedure

Resolution	48.900 - 2.200
R-factor	0.19241
Rwork	0.190
R-free	0.23083
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eih
RMSD bond length	0.007
RMSD bond angle	1.438
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.084	0.446
Number of reflections	60710	8644
<I/σ(I)>	15.2	3.9
Completeness [%]	99.7
Redundancy	6.8
CC(1/2)	0.998	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	25%(w/v) PEG3350, 0.1 M Tris-HCl (pH 8.5)