7EIH
Ancestral L-Lys oxidase (ligand free form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.441, 80.462, 173.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.300 - 2.200 |
Rwork | 0.214 |
R-free | 0.25520 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.498 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 2.310 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.106 | 0.846 |
Number of reflections | 63996 | 9129 |
<I/σ(I)> | 18.9 | |
Completeness [%] | 98.8 | |
Redundancy | 13.5 | |
CC(1/2) | 0.999 | 0.868 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 12.5% (w/v) PEG3350, 0.1 M HEPES-NaOH (pH 7.5) and 0.2 M ammonium sulfate |