7EID
Crystal structure of AF9 YEATS domain in complex with H4K8acK12ac peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.963, 44.913, 58.797 |
| Unit cell angles | 84.86, 86.09, 66.33 |
Refinement procedure
| Resolution | 39.360 - 2.000 |
| R-factor | 0.20381 |
| Rwork | 0.202 |
| R-free | 0.22881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tmp |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.996 | 2.000 |
| Number of reflections | 26432 | 1339 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 97.3 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM ammonium citrate tribasic buffer (pH 7.0) containing 19% PEG3350 |
