7EI7
RNA kink-turn motif with 2-aminopurine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.60496 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 75.934, 75.934, 41.184 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.970 - 3.200 |
R-factor | 0.251 |
Rwork | 0.245 |
R-free | 0.30450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c40 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.970 | 37.970 | 3.280 |
High resolution limit [Å] | 3.200 | 14.310 | 3.200 |
Rmerge | 0.066 | 0.028 | 0.448 |
Rmeas | 0.068 | 0.029 | 0.458 |
Total number of observations | 51123 | ||
Number of reflections | 2201 | 32 | 150 |
<I/σ(I)> | 39.55 | 86.6 | 8.76 |
Completeness [%] | 100.0 | 97 | 100 |
Redundancy | 23.227 | 16.219 | 24.613 |
CC(1/2) | 1.000 | 1.000 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium cacodylate, hexammine cobalt, MPD, strontium chloride |