7EI6

GNRA tetraloop receptor motif composed of RNA and DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.10611
Spacegroup name	H 3
Unit cell lengths	82.928, 82.928, 71.245
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.910 - 2.900
R-factor	0.1686
Rwork	0.165
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fnj
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.910	31.910	2.980
High resolution limit [Å]	2.900	12.980	2.900
Rmerge	0.068	0.035	0.273
Rmeas	0.086	0.045	0.341
Total number of observations	20875
Number of reflections	7926	83	619
<I/σ(I)>	11.1	19.07	4.12
Completeness [%]	97.8	88.3	99.5
Redundancy	2.634	2.614	2.64
CC(1/2)	0.995	0.998	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Sodium malonate pH7.0, HEPES pH7.0, Jeffamine ED-2001 pH7.0