7EHU
Chitin oligosaccharide binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-19 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.85 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.241, 69.616, 56.195 |
Unit cell angles | 90.00, 108.37, 90.00 |
Refinement procedure
Resolution | 29.150 - 1.200 |
R-factor | 0.1417 |
Rwork | 0.141 |
R-free | 0.16360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7eho |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmeas | 0.078 | 1.279 |
Rpim | 0.029 | 0.474 |
Number of reflections | 114172 | 5714 |
<I/σ(I)> | 41.6 | |
Completeness [%] | 100.0 | |
Redundancy | 7.4 | |
CC(1/2) | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG3350, MES |