7EHT
Levansucrase from Brenneria sp. EniD 312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 97.974, 97.974, 214.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.800 - 1.450 |
| Rwork | 0.172 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d47 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.800 | 1.510 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rpim | 0.028 | 0.486 |
| Number of reflections | 3547784 | 91324 |
| <I/σ(I)> | 22.2 | |
| Completeness [%] | 90.5 | |
| Redundancy | 38.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Ammounium sulfate, PEG 400 |
