7EHR
Levansucrase from Brenneria sp. EniD 312 at 1.33 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 98.000, 98.000, 213.539 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.800 - 1.330 |
| Rwork | 0.164 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d47 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.685 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.870 | 1.400 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rpim | 0.019 | 0.012 |
| Number of reflections | 138038 | 6204 |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 90.3 | 34.5 |
| Redundancy | 36.8 | 16.1 |
| CC(1/2) | 0.999 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Ammonium sulfate, PEG 400 |
