7EGL

Bicarbonate transporter complex SbtA-SbtB bound to HCO3-

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	H 3 2
Unit cell lengths	107.239, 107.239, 352.219
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.774 - 3.200
R-factor	0.2189
Rwork	0.213
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7egk
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	8.670	3.260
High resolution limit [Å]	3.200	6.890	3.200
Rmerge	0.162	0.049	1.553
Rmeas	0.171	0.052	1.642
Rpim	0.054	0.016	0.529
Number of reflections	15727	694	676
<I/σ(I)>	3.9
Completeness [%]	100.0	100	100
Redundancy	9.6	9.8	9.3
CC(1/2)		0.999	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293.15	0.05M magnesium chloride, 0.1M glycine, 22% PEG 400