7EFY

Crystal structure of retroviral protease-like domain of Ddi1 from Cryptosporidium hominis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9918
Spacegroup name	H 3 2
Unit cell lengths	81.559, 81.559, 142.181
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.439 - 2.800
Rwork	0.184
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d66
RMSD bond length	0.006
RMSD bond angle	1.497
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.260	2.800
High resolution limit [Å]	2.800	2.800
Number of reflections	4686	668
<I/σ(I)>	11.1	2.1
Completeness [%]	100.0	100
Redundancy	7.5
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	291	0.1 M MES-sodium hydroxide (pH 6.0), 30% polyacrylate sodium salt and 10% ethanol