7EFV

Crystal structure of octameric state of C-phycocyanin from Thermoleptolyngbya sp. O-77

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	I 4 3 2
Unit cell lengths	229.950, 229.950, 229.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.030 - 2.770
R-factor	0.1991
Rwork	0.196
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i7y
RMSD bond length	0.003
RMSD bond angle	0.816
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.030	2.930
High resolution limit [Å]	2.770	2.770
Rmeas	0.378	4.200
Number of reflections	26620	4259
<I/σ(I)>	11.39
Completeness [%]	100.0	100
Redundancy	61.6	61.7
CC(1/2)	0.998	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1.6 M sodium chloride, 8% polyethylene glycol 6,000, 20% glycero