7EFS

Fructose-bisphosphate aldolase in Artemisia sieversiana pollen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-12
Detector	AGILENT ATLAS CCD
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	84.007, 129.689, 157.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.490 - 2.500
R-factor	0.2119
Rwork	0.209
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rng
Data reduction software	PHENIX
Data scaling software	PHENIX
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.930	1.570
High resolution limit [Å]	1.550	1.550
Rmerge	0.119
Number of reflections	60088	1401
<I/σ(I)>	2.1
Completeness [%]	79.1
Redundancy	10.1
CC(1/2)		0.040

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5	289	12%PEG3350,0.1MNaAC