7EF9
Crystal structure of mouse MUTYH in complex with DNA containing AP site analogue:8-oxoG (Form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 71.660, 108.496, 158.527 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.530 - 1.970 |
| R-factor | 0.1788 |
| Rwork | 0.178 |
| R-free | 0.19720 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.832 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.530 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.053 | 0.576 |
| Number of reflections | 43985 | 2188 |
| <I/σ(I)> | 33.1 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350, lithium sulfate, ammonium sulfate, Bis-Tris |
