7EEO

Bulged-G motif composed of RNA, DNA and 2'-O-methyl RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.60496
Spacegroup name	P 1 21 1
Unit cell lengths	24.776, 116.432, 27.904
Unit cell angles	90.00, 107.22, 90.00

Refinement procedure

Resolution	29.108 - 2.701
R-factor	0.2128
Rwork	0.207
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q9a
RMSD bond length	0.006
RMSD bond angle	1.531
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.108	29.108	2.770
High resolution limit [Å]	2.700	12.080	2.700
Rmerge	0.054	0.032	0.267
Rmeas	0.077	0.045	0.373
Number of reflections	7521	75	555
<I/σ(I)>	8.33	14.56	2.36
Completeness [%]	90.3	78.1	85.3
Redundancy	1.565	1.52	1.564
CC(1/2)	0.994	0.993	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium chloride, MPD, hexammine cobalt, lithium chloride