7EEC

Crystal structure of EphA7 mutant G656R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-11-11
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54184
Spacegroup name	P 65
Unit cell lengths	51.780, 51.780, 216.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.843 - 3.100
Rwork	0.181
R-free	0.21010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rei
RMSD bond length	0.008
RMSD bond angle	1.589
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.030	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.175	0.175
Number of reflections	5944	874
<I/σ(I)>	11.9
Completeness [%]	100.0
Redundancy	1
CC(1/2)	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	PEG3350, ammonium sulphate, HEPES