7EEA

Cyanophage Pam1 tailspike receptor-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	123.580, 241.272, 176.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.439 - 2.671
R-factor	0.2121
Rwork	0.210
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3228)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.671	2.671
Rmerge		0.857
Number of reflections	72867	72556
<I/σ(I)>	15.7
Completeness [%]	98.5
Redundancy	12.3
CC(1/2)	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	1.34M sodium phosphate monobasic monohydrate, 0.06M potassium phosphate dibasic