7EDL

Crystal structure of the bacterial ribosomal decoding site in complex with G418 and Hg(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-23
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	31.640, 97.301, 47.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.650 - 2.600
R-factor	0.1899
Rwork	0.186
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k32
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.650	48.650	2.670
High resolution limit [Å]	2.600	11.650	2.610
Rmerge	0.055	0.030	0.336
Rmeas	0.065	0.035	0.395
Number of reflections	8525	93	631
<I/σ(I)>	15.16	29.54	3.42
Completeness [%]	99.3	95.9	99.2
Redundancy	3.516	3.14	3.593
CC(1/2)	0.998	0.998	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium cacodylate, MPD, sodium chloride, spermine tetrahydrochloride, G418, mercury(II) perchlorate